Prepare Membrane for Archiving and Re-probing Store at room temperature in a dry place.Annotate the outside of the bag with appropriate experimental information using a suitable felt tip pen.Place the membrane and filter paper in the bag and seal the bag, while removing air trapped in the bag.Place the membrane between 2 pieces of standard, lab grade filter paper.Ensure that the membrane is completely dry.Prepare Membrane for Archiving or Rescanning Only This degradation will appear as background signal in the 700 nm channel around the edges of the blot. PVDF membranes will degrade over time, due to their chemical composition.This process is likely to result in poor quality images. To re-image chemiluminescent blots that were archived without first being stripped, you must add new HRP-labeled secondary antibody and new substrate.See the Protocol section for specific instructions on how to archive blots you intend to re-probe.īlots that were stripped and re-probed cannot be used to generate quantitative results. Western Blot Storage Bags allow you to archive dry membranes.įluorescent blots or chemiluminescent blots can be rehydrated and re-probed, when appropriate. Safeguarding blots in Western Blot Storage Bags prevents scratches and protects from photobleaching for long term storage. The foil bags are lightproof and can accommodate standard size minigel blots (7 x 8cm) up to Criterion™ gel blots (8.5 x 13.5cm). ![]() Western Blot Storage Bags are a convenient way to save, store, and organize Western blot membranes. Western Blot Storage Bags 40 bags per pack
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